The curse pronounced in Genesis 3:15 after Adam and Eve sinned establishes enmity not only between Eve and Satan but also between the seed of Satan and the seed of Eve. This judgment initiates a separation among people that God later reinforces through sanctification. The seed of Satan consists of those whom he influences spiritually so that they bear his characteristics, as seen in Cain and Lamech, while the seed of Eve includes the line descending through Seth. God places a fear of Satan in those descended from Adam and Eve through Seth, creating a protective boundary that keeps His people from drawing near to the destroyer of all that is good. This enmity supplies the us-and-them division God wills for the benefit of the called, giving them something to resist and testing their faithfulness. The same curse supplies the foundation for the doctrine of sanctification, by which God separates individuals such as Noah and his family from the rest of humanity, granting them grace and protection during the Flood while the rest perish. Sanctification thus flows directly from the curse, marking the initial separation that only God performs and beginning the process by which the separated become holy in nature through the new creation. This work prepares a people to fulfill responsibilities in God's purpose of forming a family in His image, ensuring that those He calls remain distinct and equipped to resist the influence of the wicked one who holds sway over the world.